{"product_id":"perforin-polyclonal-antibody-bs91051","title":"Perforin Polyclonal Antibody-BS91051","description":"\u003cp\u003ePerforin Polyclonal Antibody\u003c\/p\u003e\n\n\u003cp\u003eSizes: 50µl, 100µl\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: BS91051-50, BS91051-100\u003c\/p\u003e\n\n\u003cp\u003eProduct: Rabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/p\u003e\n\n\u003cp\u003eSwiss-Prot: P14222 Human\u003c\/p\u003e\n\n\u003cp\u003eHost: Rabbit\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eApplications: WB, ICC IHC FC\u003c\/p\u003e\n\n\u003cp\u003eAll Applications: WB:1:500-1:2,000\u003cbr\u003e\nICC:1:50-1:200\u003cbr\u003e\nIHC:1:50-1:200\u003cbr\u003e\nFC:1:50-1:100\u003c\/p\u003e\n\n\u003cp\u003eBackground: The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores.\u003c\/p\u003e\n\n\u003cp\u003ePurification and Purity: Protein affinity purified.\u003c\/p\u003e\n\n\u003cp\u003eStorage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze \/ thaw cycles.\u003c\/p\u003e\n\n\u003cp\u003eSpecificity: Perforin polyclonal antibody detects endogenous levels of Perforin protein.\u003c\/p\u003e\n\n\u003cp\u003eBioworld Molecular Weight: 90 kDa, predicted band size 61 kDa\u003c\/p\u003e\n\n\u003cp\u003eNote: For research use only, not for use in diagnostic procedure.\u003c\/p\u003e\n\n\u003cp\u003eExtra Notes: Western blot analysis of Perforin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.\u003cbr\u003e\n Positive control: \u003cbr\u003e\n Lane 1: HepG2 cell lysate\u003cbr\u003e\n Lane 2: Daudi cell lysate, ICC staining Perforin in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Perforin polyclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc and trade; 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1\/100 dilution. The nuclear counter stain is DAPI (blue).\u003c\/p\u003e\n\n\u003cp\u003eAlternative Name: P1, PFP, PRF1, Cytolysin, FLH2, HPLH2, Lymphocyte pore-forming protein, P1, PERF, perforin 1 (pore forming protein), Perforin 1, Perforin-1, PFP, PGFL, PIGF, PIGF-2, PLGF, Pore forming protein, prf1, SHGC-10760 antibody\u003c\/p\u003e\n\n\u003cp\u003eImmunogen: Recombinant protein within Human Perforin aa 300-500.\u003c\/p\u003e\n\n\u003cp\u003eConjugate: Unconjugated\u003c\/p\u003e\n\n\u003cp\u003eModification: Unmodified\u003c\/p\u003e","brand":"BioWorld","offers":[{"title":"50µl","offer_id":45296238690486,"sku":"BS91051-50","price":241.32,"currency_code":"USD","in_stock":true},{"title":"100µl","offer_id":45296238723254,"sku":"BS91051-100","price":372.28,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0731\/8251\/1286\/files\/thumbnail_d1447bac803ec9be_11-14-09-19-51_0fc59d1a-dabf-489e-8cd9-81d7eb33e46c.jpg?v=1785260909","url":"https:\/\/mhkshopmotfy.com\/products\/perforin-polyclonal-antibody-bs91051","provider":"Mhkshopmotfy","version":"1.0","type":"link"}